Iridian, the leader in optical filter solutions, now offers ultra performance filters specially designed for Real Time PCR (polymerase chain reaction) applications. Our standard filter solutions are compatible with common excitation sources and fluorophores but we specialize in customized filter solutions. The ultra PCR fluorescence filter sets are designed to provide a high signal-to-noise ratio of the probes contained in Real-Time PCR samples when illuminated with a LED light source.
Iridian’s emission, excitation and dichroic filters provide more signal with less background to the fluorescence instrument users and OEMs.
Dichroic filters
- Tx >95% average for the passband
- Reflection >97% over the reflection range
- AOI: 45 +/- 2 degrees; CHA (cone half angle) 5 degrees
- Edge Steepness < 1% (10%T – 90%T)
- Wavelength Range: 350-850 nm
- Custom sizing available
| Fluorochrome | Filter Name |
|---|---|
| FAM Channel | PCR 505nm DLP |
| HEX Channel | PCR 550nm DLP |
| ROX Channel | PCR 590nm DLP |
| Cy5 Channel | PCR 647nm DLP |
| Cy5.5 Channel | PCR 700nm DLP |
HEX/VIC/JOE Channel:
EX Filter: 530-20nm; Dichroic: 550DLP; EM Filter: 565-22nm
Excitation and Emission Filters
- Tx >95% average for the passband
- Blocking > OD6 outside passband
- AOI: 0 – 5 degrees; CHA 5 degrees
- Edge Steepness < 0.5% (50%Tmax – OD5)
- Wavelength Range: 350-850 nm
- Custom sizing available
| Fluorochrome | Filter Name |
|---|---|
| FAM Channel | 470-30nm EX /520-20nm EM |
| HEX Channel | 530-20nm EX/565-22nm EM |
| ROX Channel | 570-20nm EX/615-40nm EM |
| Cy5 Channel | 630-20nm EX/665-20nm EM |
| Cy5.5 Channel | 685-20nm EX/725-40nm EM |
